Microbial cells as catalysts for stereoselective red-ox reactions - Biotechnology Advances 2009

J.D. Carballeira, M.A. Quezada, P. Hoyos, Y. Simeó, M.J. Hernaiz, A.R. Alcantara and J.V. Sinisterra.
- Industrial Biotransformations Service, Scientific Park of Madrid, C/ Santiago Grisolía n° 2, Tres Cantos, 28760 Madrid, Spain
- Molecular Interactions Service, Scientific Park of Madrid, Faculty of Pharmacy, Universidad Complutense, 28040 Madrid, Spain
- Biotransformations Group, Department of Organic & Pharmaceutical Chemistry, Faculty of Pharmacy, Universidad Complutense, 28040 Madrid, Spain

Enzyme catalyzed reactions are commonly used at laboratory or industrial scale. Contrarily, the whole cells catalyzed reactions are restricted to special cases. The tremendous advances in the last years in Molecular Biology and more specifically in Metabolic Engineering and Directed Enzyme Evolution have opened the door to create tailor-made microorganisms or “designer bugs” for industrial purposes. Whole cells catalysts can be much more readily and inexpensively prepared than purified enzymes and the enzymes - inside the cells – are protected from the external environment and stabilized by the intracellular medium. Three situations have traditionally been considered convenient to select the use of whole cell catalyzed processes against the free enzyme catalyzed process: i) when the enzyme is intracellular; ii) when the enzyme needs a cofactor to carry out the catalytic act and iii) in the development of multi-enzymatic processes. Redox reactions represent the molecular basis for energy generation in the cell. These reactions are catalyzed by intracellular enzymes and are cofactor dependent as redox reactions need electron carriers as helpers in reduction reactions (gain of electrons) or oxidation (loss of electrons).
In this review we present an overview of the state of the art of red-ox biotransformations catalyzed by whole cells - wild-type or genetically engineered microorganisms. Stereoselective reductions, hydroxylations of arenes and unfunctionalized alkanes, alkene monooxygenation, Baeyer-Villiger reactions are among the processes described along the text, focusing in their chemo-, regio- and stereoselectivity.
Keywords: Biotransformations; Biocatalysis; Whole-cell-catalysts; Cell-factories; Designer-bugs Immobilization of cells; stereoselective reactions; red-ox reactions

http://dx.doi.org/10.1016/j.biotechadv.2009.05.001

Efficient Horner-Wadsworth-Emmons intramolecular cyclisation of a N-substituted phthalimide promoted by KF-Alumina: a general tool for the synthesis o

Vittorio Pace, Fernando Martınez, Clara I. Nova, Marıa Fernández, José Vicente Sinisterra and Andrés R. Alcántara

Department of Organic and Pharmaceutical Chemistry, Pharmacy Faculty, Complutense University, Pza. Ramón y Cajal s/n, 28040 Madrid Spain

Industrial Biotransformations Service (SBI), Scientific Park of Madrid (PTM), C/ Santiago Grisolia 2, 28760 Tres Cantos, Madrid Spain

Abstract
An intramolecular Horner-Wadsworth-Emmons reaction promoted by KF-Alumina involving a N-substituted phthalimide, cleanly and efficiently furnishes an interesting α,β-unsaturated tricyclic enone which may undergo selective alkylations at the α’-position.

http://dx.doi.org/10.1016/j.tetlet.2009.04.029

Growth hormone improves lipoprotein concentration and arylesterase activity in mice with an atherogenic lipid profile induced by lactalbumin

E. LOPEZ-OLIVA, M. NUS, A. AGIS-TORRES, W. VILLARO, J.M. SANCHEZ-MONTERO, E. MUÑOZ-MARTINEZ, F.J. SANCHEZ-MUÑIZ

Sección Departamental de Fisiología Animal, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain

Departamento de Nutrición y Bromatología I (Nutrición), Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain

Biotransformations Group, Departamento de Química Orgánica y Farmacéutica, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain

The effect of growth hormone (GH) on arylesterase (AE), one of the activities of paraoxonase, has never been studied. The aims of the present study in mice were: (a) to compare the effect of age and sex on serum lipid and lipoprotein levels after consumption of lactalbumin- v. chow-based diets and (b) to study the effect of GH administration, age and sex on serum AE activity, lipid and lipoprotein and body fat levels in mice fed a lactalbumin diet. Seventy-two mice were divided into three age- and sex-matched experimental groups: (1) control chow (CC), (2) non-GH lactalbumin (NGL) and (3) GH-treated lactalbumin (GL) mice. Lactalbumin increased total cholesterol, (LDL+VLDL)-cholesterol and TAG and diminished HDL-cholesterol in all animals

http://dx.doi.org/10.1017/S0007114508025014

A global benchmark study using affinity-based biosensors

Prof. MJ Hernaiz is one of the co-authors of this global study

To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amenable to analysis using different biosensor platforms as well as by users of different expertise levels. The two proteins (a 50-kDa Fab and a 60-kDa glutathione S-transferase [GST] antigen) form a relatively high-affinity complex, so participants needed to optimize several experimental parameters, including ligand immobilization and regeneration conditions as well as analyte concentrations and injection/dissociation times. Although most participants collected binding responses that could be fit to yield kinetic parameters, the quality of a few data sets could have been improved by optimizing the assay design. Once these outliers were removed, the average reported affinity across the remaining panel of participants was 620 pM with a standard deviation of 980 pM. These results demonstrate that when this biosensor assay was designed and executed appropriately, the reported rate constants were consistent, and independent of which protein was immobilized and which biosensor was used.
Keywords: Biacore; Kinetics; Optical biosensor; Surface plasmon resonance

http://dx.doi.org/10.1016/j.ab.2008.11.021

Glycan Tagging to Produce Bioactive Ligands for a Surface Plasmon Resonance (SPR) Study via Immobilization on Different Surfaces.

F.J MUÑOZ, J. PEREZ, A. RUMBERO, J.I. SANTOS, F.J. CAÑADA, S. ANDRE, H.J. GABIUS, J. JIMENEZ-BARBAERO, J.V. SINISTERRA, M.J. HERNAIZ.

Departamento de Quimica Organica y Farmaceutica, Universidad Complutense de Madrid, Plaza Ramon y Cajal s/n. 28040 Madrid, Spain
Departamento de Quimica Organica, Universidad Autonoma de Madrid, Campus de Cantoblanco, 28049 Madrid, Spain

Departamento de Ciencia de Proteinas, CIB-CSIC, c/Ramiro de Maeztu 9, 28040 Madrid, Spain, Institut fur Physiologische Chemie, Tierarztliche Fakultat, Ludwig-Maximilians-Universitat, Munchen, Veterinarstr 13, 80539 Munchen, Germany

Servicio de Biotransformaciones Industriales, Parque Cientifico de Madrid C/Santiago Grisolia, 28760 Tres Cantos, Spain & Servicio de Interacciones Biomoleculares, Parque Cientifico de Madrid, Pz/Ramon y Cajal s/n. 28040 Madrid, Spain.

Suitable glycan derivatives will find immediate application in the study of their interactions. Here, we present an efficient synthetic strategy to introduce a fluorescent tag functionalized with an amino group into a model disaccharide structure (lactose). This strategy led to the maintenance of bioactivity, checked by the study of the interaction of this bioconjugate with a plant lectin (mistletoe lectin 1) by NMR spectroscopy, computational docking, and surface plasmon resonance (SPR). To demonstrate the versatility of this approach, we immobilized the new glycan derivatives on different surfaces, and a comparative analysis is presented and can be successfully used for biomimetic carbohydrate-protein interaction studies on the SPR biochip.

http://dx.doi.org/10.1021/bc800350q

Biotransformations. vol. 1, pp. 212-251. Encyclopedia of Microbiology 3rd Edition. Oxford. Elsevier

J.D. CARBALLEIRA, J. FERNANDEZ-LUCAS, M.A. QUEZADA, M.J. HERNAIZ, A.R. ALCANTARA, Y. SIMEO, J.V. SINISTERRA

The application of whole-cell-catalyzed biotransformations is an emerging field that opens up a myriad of new possibilities for the industrial application of biocatalysts. In this article, the new technologies to design tailor-made cells for specific reactions as well as some interesting applied processes catalyzed by intracellular enzymes are presented.

Encyclopedia of Microbiology
Moselio Schaechter, San Diego State University, CA, USA
Six-Volume Set, 1-6 Hardbound, 4600 pages, publication date: APR-2009
ISBN-13: 978-0-12-373939-1
ISBN-10: 0-12-373939-X
Imprint: ACADEMIC PRESS

Monascus kaoliang CBS 302.78 immobilized in polyurethane foam using iso-propanol as co-substrate: Optimized immobilization conditions of a fungus as b

M.A. Quezada, J.D. Carballeira and J.V. Sinisterra

Department of Chemistry, Faculty of Chemical Engineering, University of Trujillo, Peru
Industrial Biotransformations Service, Scientific Park of Madrid, C/Santiago Grisolia No. 2, Parque Tecnológico de Madrid, 28760 Tres Cantos, Madrid, Spain

Biotransformations Group, Organic and Pharmaceutical Chemistry Department, Faculty of Pharmacy, Universidad Complutense, 28040 Madrid, Spain

Abstract
Monascus kaoliang was selected after a microbial screening as a highly active and selective whole cell catalyst for the reduction of ketones. In the present paper we describe the optimum growing conditions and an interesting immobilization procedure by adsorption in polyurethane foams (PUFs). This methodology is easy to perform and the immobilized catalyst is active, stable and reusable. The use of different co-substrates for cofactor regeneration was also tested and iso-propanol (i-PrOH) was found as the best co-substrate, as it leads to a catalyst reusable for 17 cycles, displaying better NADH regeneration properties than others e.g., glucose (10 cycles) or saccharose (6 cycles). The reduction of different prochiral ketones showed that the ketone reductase activity of this mould follows the Prelog’s rule and kinetic experiments demonstrated that the process follows a pseudo-first kinetic order.

Biotransformation of Terpenoids: A Green Alternative for Producing Molecules with Pharmacological Activity

Y. Simeó and J.V. Sinisterra

Mini Reviews in Organic Chemistry 2009

Terpenoids are natural products of great interest due to their broad application scope. They are employed as agrochemicals, drugs, fragrances, flavours and pigments. In the search of new derivatives with improved properties, the use of biocatalysts is being constantly increased, especially in redox processes. They can give rise to stereo- and regioselective products and/or compounds functionalized in remote positions difficult to reach by means of traditional organic chemistry. In this review, the application of whole cell catalyzed biotransformations of terpenoids to obtain new drug targets or to increase the pharmacological activity is presented.

http://www.bentham.org/mroc/

Defining the Epitope Region of a Peptide from the Streptomyces coelicolor Phosphoenolpyruvate:Sugar Phosphotransferase System Able to Bind to the Enzy

E. HURTADO-GOMEZ, O. ABIAN, F. J. MUÑOZ , M.J. HERNAIZ, A. VELAZQUEZ-CAMPOY, J.L. NEIRA

Abstract
The bacterial PEP:sugar PTS consists of a cascade of several proteins involved in the uptake and phosphorylation of carbohydrates, and in signal transduction pathways. Its uniqueness in bacteria makes the PTS a target for new antibacterial drugs. These drugs can be obtained from peptides or protein fragments able to interfere with the first reaction of the protein cascade: the phosphorylation of the HPr by the first enzyme, the so-called enzyme EI. To that end, we designed a peptide, HPr9–30, spanning residues 9 to 30 of the intact HPr protein, containing the active site histidine (His-15) and the first α-helix of HPr of Streptomyces coelicolor, HPrsc. By using fluorescence and circular dichroism, we first determined qualitatively that HPrsc and HPr9–30 did bind to EIsc, the enzyme EI from S. coelicolor. Then, we determined quantitatively the binding affinities of HPr9–30 and HPrsc for EIsc by using ITC and STD-NMR. The STD-NMR experiments indicate that the epitope region of HPr9–30 was formed by residues Leu-14, His-15, Ile-21, and Val-23. The binding reaction between EIsc and HPrsc is enthalpy driven and in other species is entropy driven; further, the affinity of HPrsc for EIsc was smaller than in other species. However, the affinity of HPr9–30 for EIsc was only moderately lower than that of EIsc for HPrsc, suggesting that this peptide could be considered a promising hit compound for designing new inhibitors against the PTS.
Abbreviations: PEP, phosphoenolpyruvate; CD, circular dichroism; EIsc, enzyme I of Streptomyces coelicolor; EIec, enzyme I of E. coli; HPrec, histidine phosphocarrier protein of Escherichia coli; HPrsc, histidine phosphocarrier protein of Streptomyces coelicolor; HPr9-30, peptide comprising residues Gly-9 to Gly-30 of the intact HPrsc; ITC, isothermal titration calorimetry; NMR, nuclear magnetic resonance; PTS, sugar phosphotransferase system; STD, saturation transfer difference; TFE, trifluoroethanol; TSP, sodium trimethylsilyl [2,2,3,3-2H4] propionate; UV, ultraviolet

http://dx.doi.org/10.1529/biophysj.107.126664

Versatile strategy for the synthesis of biotin-labelled glycans, their immobilization to establish a bioactive surface and interaction studies with a

F.J MUÑOZ, A. RUMBERO, J.V. SINISTERRA, J.I. SANTOS, S. ANDRE, H.J. GABIUS, J. JIMENEZ-BARBERO, M.J. HERNAIZ

Abstract The emerging role of glycans as versatile biochemical signals in diverse aspects of cellular sociology calls for establishment of sensitive methods to monitor carbohydrate recognition by receptors such as lectins. Most of these techniques involve the immobilization of one of the binding partners on a surface, e.g. atomic force microscopy, glycan array and Surface Plasmon Resonance (SPR), hereby simulating cell surface presentation. Here, we report the synthesis of fluorescent glycoconjugates, with a functionalization strategy which avoids the frequently occurring ring opening at the reducing end for further immobilization on a surface or derivatization with biotin. In order to improve the versatility of these derivatized glycans for biological studies, a new approach for the synthesis of biotinylated and fluorescent glycans has also been realized. Finally, to illustrate their usefulness the neoglycoconjugates were immobilized on different surfaces, and the interaction analysis with a model lectin, the toxin from mistletoe, proved them to act as potent ligands, underscoring the merit of the presented synthetic approach.
Electronic supplementary material The online version of this article (doi:10.1007/s10719-008-9115-y) contains supplementary material, which is available to authorized users.

http://dx.doi.org/10.1007/s10719-008-9115-y

Esterification reactions catalyzed by lipases immobilized in organogels: effect of temperature and substrate diffusion

M. ZOUMPANIOTI, P. PARMAKLIS, P. DOMINGUEZ DE MARIA, H. STAMATIS, J.V. SINISTERRA, A. XENAKIS

Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece

Biotransformations Group, Faculty of Pharmacy, Universidad Complutense, Madrid, Spain

Biological Applications and Technologies Department, University of Ioannina, Ioannina, Greece

Abstract

Rhizomucor miehei lipase was immobilized in hydroxy(propylmethyl) cellulose or agar gels containing lecithin or AOT microemulsions. The effect of the diffusion of substrates and products to this catalyst was studied, as well as the effect of temperature on the initial rate of ester synthesis. The composition of the gel affects the reaction rate due to mass transport phenomena. The apparent activation energies were higher for the systems based on agar, independently of the microemulsion used, and lower for the systems based on AOT microemulsions, independently of the polymer used.

http://dx.doi.org/10.1007/s10529-008-9734-1

Low temperature synthesis of 2-deoxyadenosine using immobilized psychrotrophic microorganisms

J. FERNANDEZ-LUCAS, L.A. CONDEZO, M.A. QUEZADA and J.V. SINISTERRA

Keywords
nucleoside 2-deoxyribosyl-transferases • synthesis of 2-deoxyribosyl nucleosides • psychrotrophic microorganisms • immobilized whole cells • calcium pectate • calcium alginate
Abstract
Selective biocatalyzed synthesis of 2-deoxyadenosine from 2-deoxypyrimidine nucleosides was carried out using free or immobilized whole cells. The reaction was performed at 57°C without secondary reactions. Two psychrotrophic microorganisms, Bacillus psychrosaccharolyticus and Psychrobacter immobilis, are described for the first time as active and specific strains for the synthesis of 2-deoxyadenosine. Adenosine deaminase activity was not detected. Whole cells were immobilized in different matrixes. Calcium alginate and calcium pectate gave the best biocatalysts. The synthesis of 2-deoxyadenosine follows an apparent first order kinetic expression. External mass transfer control was negligible as deduced from ks, NA, and values. Internal mass transfer was the rate controlling step according to T and values. Biotechnol. Bioeng. 2008;100: 213-222. © 2007 Wiley Periodicals, Inc.

http://dx.doi.org/10.1002/bit.21756

Prof. José Vicente Sinisterra en la Universidad de Trujillo - Perú

JUEVES 6 DE NOVIEMBRE DE 2008


ESPECIALISTA DE UNIVERSIDAD COMPLUTENSE ARRIBA A UNIVERSIDAD NACIONAL DE TRUJILLO

Dr. José Sinisterra Gago ganó proyecto en Europa de 31 mil euros para ejecutarlo en Trujillo. El ilustre visitante español es a la vez director de la Bioincubadora de empresas del parque Científico de Madrid; director del Grupo de Biotransformaciones de la Universidad Complutense de Madrid; y un investigador de gran nivel.El catedrático del departamento de Química Orgánica y Farmacéutica de laUniversidad Complutense de Madrid (España), Dr. José Vicente Sinisterra Gago, acaba de llegar a Trujillo para dictar desde este lunes 9 de noviembre un curso de tres semanas de duración dirigido a docentes y alumnos de la Facultad de Ingeniería Química de la Universidad Nacional de Trujillo.El ilustre visitante español es a la vez director de la Bioincubadora de empresas delparque Científico de Madrid; director del Grupo de Biotransformaciones de la Universidad Complutense de Madrid; y un investigador de gran nivel que ha participado en diversos proyectos financiados por la Unión Europea; así como autor de diversas publicaciones en revistas internacionales y diversas patentes.El rector de la UNT, Dr. Víctor Carlos Sabana Gamarra se mostró complacido con esta nueva visita, pues recordó que el científico español visitó la UNT el año pasado donde participó en cursos y seminarios dirigidos a profesores de esta Casa Superior de Estudios. “Pero ahora nos trae una feliz noticia, pues en su identificación con la UNT presentó en Madrid un proyecto para capacitar a nuestros docentes en temas de química. Ahora nos cuenta que ha ganado una subvención de 31 mil 500 euros para hacer realidad su proyecto”, reveló.El Dr. Sinisterra Gago informó que el curso se inaugura este lunes 9 de noviembre y abordará los temas de “Biotransformaciones”, “Análisis y control de calidad”y “Validación de métodos analíticos por CG-MS y HPLC”. El proyecto“Formación de profesorado de la Facultad de Ingeniería Química de la Universidad de Trujillo (UNT)” fue seleccionado entre 100 solicitudes en la Quinta Convocatoria de proyectos a ser financiados por la Universidad Complutense de Madrid y dotado con una subvención de 31 mil euros, en colaboración con Agencia de Cooperación de Iberoamérica y el Banco de Santander.El especialista explicó que las Biotransformaciones son reacciones químicas que ocurren en condiciones no contaminantes, para evitar la contaminación del planeta; es decir cambiar los reactivos químicos convencionales que son contaminantes por otros menos contaminantes. “En España a partir de julio 2008 es obligatorio trabajar con disolventes no contaminantes, no se acepta ningún desarrollo industrial con disolventes contaminantes; en Perú estuve 8 años trabajando en Tingo María, donde monté un laboratorio de investigación y desarrollo y estamos bastante bien; pero la costa aún está muy contaminada”, expresó.En ese sentido, propuso iniciar un trabajo sobre análisis y control de calidad y asesoría a través de la Universidad Nacional de Trujillo sobre contaminación de metales, de productos orgánicos, de pesticidas que se usan en el proyecto especial Chavimochic. Igualmente, se montará dentro del Departamento de Química un laboratorio de Control de Calidad para que puedan hacer trabajos de asesoría a las empresas agroexportadoras de la región La Libertad.

BIOCAT 2008

Three oral communications from the Biotransformations Group:

- Josep V. Sinisterra, Complutense University of Madrid, Madrid, Spain "New Microorganisms Active in the Production of Enzymes with Industrial Interest"
- Andres R. Alcántara, Complutense University of Madrid, Madrid, Spain "Chemoenzymatic Synthesis of Homochiral α-Hydroxyketones by Two Biocatalytical Approaches"
- Maria J. Hernáiz, Complutense University of Madrid, Madrid, Spain "Enzymatic Approaches in the Preparation of Oligosaccharides and Glycoconjugates"

http://www.biocat2008.de/

Doctorado de Química Sostenible con mención de calidad

Coordinador principal en la Universidad Complutense 2008-2009: Prof. José María Sánchez Montero (Grupo de Biotransformaciones)

http://www.ucm.es/pags.php?tp=Estudios%20de%20Doctorado&a=estudios&d=0001463.php

Oficina Erasmus- Sócrates Facultad de Farmacia

El Prof. Andrés Alcántara León del Grupo de Biotransformaciones has sido nombrado responsable de la Oficina Erasmus de la Facultad de Farmacia de la Universidad Complutense de Madrid.

http://www.ucm.es/info/farmacia/Erasmus.htm

ESF-EMBO Symposium - PROTEIN DESIGN AND EVOLUTION FOR BIOCATALYSIS

The meeting will bring together internationally renowned speakers who will review the current state of knowledge in the field of protein design and evolution for biocatalysis. The symposium will be the third event in a series held in Paris (2002) and Greifswald (2006). The main objective of the 2008 meeting will be to intensify discussion and collaboration between experimentalists with theoreticians, which is needed for development of novel biocatalysts by combining directed evolution with rational protein design.

http://www.esf.org/index.php?id=4569

Biotec 2008

The Biotec2008 Congress on Biotechnology organized by the Spanish Society of Biotechnology (SEBIOT) has been designed to continue the tradition of previous Biotec meetings in order to bring together the Spanish top and young scientists working in Biotechnology. The Congress will be held in Granada from the 17th to the 19th of September 2008 on the scenario provided by the BioSpain08 meeting organized by ASEBIO (Spanish Association of Bioenterprises) where biotechnological companies, innovators and key biotech policy and decision makers will share their current experience and future innovations. This joint venture between SEBIOT and ASEBIO was established for the first time two years ago for the BiosSpain-Biotec2006 Congress (Madrid) sponsored by the Fundación Genoma España, with the objective of combining the biotechnological aims of academy and industry. The previous edition attracted over 1,200 leading scientists and other senior participants, over 300 posters and more than 50 exhibitors.
The BIOTEC2008 aims to bring together researchers from academy and industry in the field of Biotechnology.
Scientific topics of this BIOTEC edition are:
- Applied Biocatalysis
- Biochemical Engineering
- Environmental Biotechnology
- Biotechnology and Health
- Plant Biotechnology
- Food Biotechnology
- Microbial Biotechnology
- Biotechnology and Society
- Bioinformatics

http://www.sebiot.org/biotec/ing/index.htm

"Primer Curso Iberoamericano de Biocatálisis aplicada a la Química Verde (CIBaQ)"

Participa como Docente el Director del Grupo de Biotransformaciones de la Universidad Complutense de Madrid y del Servicio de Biotransformaciones Industriales del Parque Científico de Madrid, Prof. Dr. José Vicente Sinisterra Gago

Del 8-12 de Septiembre de 2008.

Universidad Nacional de Quilmes. Argentina. http://www.unq.edu.ar/

Información sobre el Curso http://sites.google.com/site/cibaqu/

Oferta de empleo en el Grupo de Biotransformaciones

Solicitud Contrato de Personal Investigador de Apoyo convocatoria CAM

TÉCNICAS EXPERIMENTALES EN EL DISEÑO DE FÁRMACOS MEDIANTE BIOTRANSFORMACIONES. Curso de Verano UCM.

Curso enmarcado en la escuela de medio ambiente y tecnología
Directores: Dr. D. José María Sánchez Montero y Dr. D. Andrés Rafael Alcántara León.
Fechas y horario: Del 3 al 31 de julio de 2008. Mañanas de 9:00 a 14:00 horas, de lunes a viernes.
Nº de plazas: 20
Nº de créditos de libre configuración: 8 (sólo alumnos UCM).
Lugar de celebración: Universidad Complutense de Madrid.
Precio de la matrícula: 900 Euros
Ayudas: Las de caracter general
Perfil del alumno: El curso va especialmente dirigido a estudiantes, diplomados o licenciados con relación, o interés, en temas de Biotecnología. En concreto, el curso puede ser de especial interés para estudiantes o licenciados en, Farmacia, Biológicas, Químicas, e Ingenieros Químicos.
Más información: Programa completo del curso

http://www.ucm.es/info/fgu/escuelas/verano/cursos/i11.html

JORNADA SOBRE BIOTECNOLOGÍA EN ESPAÑA: VISION ACTUAL

Curso del plan de formación del Parque Científico de Madrid. Unidad de Biotransformaciones Industriales del Parque Científico de Madrid. C/Santiago Grisolía, 2 Parque Tecnológico de Madrid

Noticias

- Madri+d. Biotransformaciones en los Fármacos. Jose María Sánchez Montero & Andrés Alcántara. "La implantación de la Biotecnología Blanca o Biotecnología Industrial en el sector farmacéutico..." [Artículo].

- Curso Práctico: Cromatografía HPLC para la Industria Alimentaria, Farmacéutica y Química Fina. Universidad Nacional Agraria de la Selva. Facultad de Ingeniería en Industrias Alimentarias. Del 5-9 de Noviembre de 2007. Prof. Dr. Jose Vicente Sinisterra Gago. [Información]

- Curso Práctico de Tecnología Farmacéutica sobre formulaciones de aplicación cutánea. Coordinador: Dra. Amalia Rodriguez Bayón (UCM-PCM). Unidad de Biotransformaciones Industriales. Parque Científico de Madrid. 12-16 de Noviembre de 2007. [info]

- Curso Práctico de Cromatografía de Gases. Coordinador: Prof. Dr. José Vicente Sinisterra Gago (UCM-PCM). Unidad de Biotransformaciones Industriales. Parque Científico de Madrid. 16-17 de Octubre 2007 [info]

- Curso Práctico de Tecnología Farmacéutica sobre solubilidad y solubilización de fármacos. Coordinador: Dra. Amalia Rodriguez Bayón (UCM-PCM). Unidad de Biotransformaciones Industriales. Parque Científico de Madrid. 23-26 de Octubre 2007. [info]

- 04/06/2007 New Biotin reagent patented. Madri+d

- 20/03/2007 Tribuna Complutense: Industrial Biotransformations Unit - Scientific Park of Madrid.

- 06/03/2007 Tribuna Complutense: Cinfa Prize goes to BTg Researchers

Dynamic Kinetic Resolution of Benzoins by Lipase-Metal Combo Catalysis

P. HOYOS, M. FERNANDEZ, J.V. SINISTERRA, A.R. ALCANTARA

The synthesis of some noncommercial racemic 1,2-diaryl-2-hydroxyethanones (benzoins) is described, optimizing the previously reported methodologies. In a further step, the kinetic resolution of these substrates is reported, obtaining conversions of around 50% and eep higher than 99% in very short reaction times. As enzymatic catalyst, after screening of several enzymes, the lipase TL (from Pseudomonas stutzeri) was the most efficient, working in an organic solvent with a very low log P value, such as THF. Finally, the dynamic−kinetic resolution of different benzoins using a lipase−ruthenium-catalyzed transesterification in organic solvents is described for the first time, obtaining conversions up to 90% maintaining the excellent enantioselectivity in all cases.

http://dx.doi.org/10.1021/jo061060b

Effective monoallylation of anilines catalyzed by supported KF

V. PACE, F. MARTINEZ, M. FERNANDEZ, J.V. SINISTERRA, A.R. ALCANTARA

Abstract
A mild and straightforward monoallylation procedure for different anilines is described using the efficient, inexpensive, noncorrosive, and environmentally friendly reagent KF-Celite. By using only a 1/1.2 stoichiometric ratio of electrophilic reagent to aniline, in very short reaction times, the monoallylated products are obtained in high isolated yields via this procedure, which works very effectively regardless of the electronic nature of the substituent on the ring, although electron withdrawing groups make the reactions go even faster.

http://dx.doi.org/10.1021/ol070890o