Green glycosylation using ionic liquid to prepare alkyl glycosides for studying carbohydrate–protein interactions by SPR

F. J. MUÑOZ, S. ANDRE, H.J. GABIUS, J.V. SINISTERRA, M.J. HERNAIZ, R.J. LINHARDT

Green Chemistry, 2009, 11, 373 - 379

Abstract

Several simple glycosides of D-glucose (Glc) and N-acetyl-D-galactosamine (GalNAc) were prepared in a single step glycosylation reaction using unprotected and unactivated sugar donors. The resulting GalNAc glycoside, containing a bifunctional linker, was used to immobilize this glycoconjugate to a self assembled monolayer on a gold biosensor chip. Surface plasmon resonance (SPR) experiments demonstrated that this immobilized glycoconjugate bound to GalNAc specific lectin, Viscum album agglutinin.

http://dx.doi.org/10.1039/b814171a